We narrowed to 5,632 results for: chia

1. pSF-p15A-T7- YFP

   Plasmid

   #186568

   Purpose

   p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE1069

   Plasmid

   #186629

   Purpose

   SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

   Inserts

   scbR
   scbAp promoter
   

   Use

   Synthetic Biology

   Tags

   6xArg

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23100 and scbAp

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDR111_mannose_Bgal NLS

   Plasmid

   #188398

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB101

   Plasmid

   #169110

   Purpose

   Feedback-controlled AraC circuit

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   araC (araC Escherichia coli K12 MG1655)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB200

   Plasmid

   #169111

   Purpose

   TESEC backbone for target expression

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   lacZ (lacZ Escherichia coli K12 MG1655)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBN216

   Plasmid

   #167963

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBN217

   Plasmid

   #167964

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN262

   Plasmid

   #167967

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBRC901

   Plasmid

   #167948

   Purpose

   express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBN202

    Plasmid

    #167950

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4LNDD-pCDNA3.1(+)

    Plasmid

    #172227

    Purpose

    Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKP1693

    Plasmid

    #167595

    Purpose

    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

    Depositor

    Kathleen Postle

    Article

    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

    Insert

    FepA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His6 tag inserted after residue 393 in the mature…

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-null (BPK1077)

    Plasmid

    #171128

    Purpose

    Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKB238

    Plasmid

    #170033

    Purpose

    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Neuropeptide Y (NPY Human, Synthetic, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKB235

    Plasmid

    #170024

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 (CCL20 Human, Synthetic, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid

    #160890

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-S1H
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-SH1: T7, chaperones: T13

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid

    #160887

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T4a: T7, chaperones: T10

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge