We narrowed to 77,003 results for: rest
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Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTMP
Plasmid#129560Purposecytoplasmic markerDepositorInsertmCherry
ExpressionBacterialAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-EcSsra
Plasmid#131217PurposeExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-EcSsra
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-pep
Plasmid#131215PurposeExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-pep
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
TagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL800
Plasmid#119306Purposeprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290SDepositorInsertprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
TagsnoneExpressionBacterialMutationamino acids 1 through 171 deleted, and mutatioms …PromoterlacAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
TagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-MCS-Avi
Plasmid#110205PurposeRecipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choiceDepositorInsertENH_BsmB1-MCS-Avi
TagsAvi_tagExpressionBacterialAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-Avi-MCS-2A-Citrine
Plasmid#110204PurposeRecipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.DepositorInsertENH_BsmB1-Avi-MCS-2A-Citrine
TagsAvi_tag-2a-YFPExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (220aa)
Plasmid#45287DepositorAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-151)
Plasmid#37308DepositorAvailable SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-REVERBA-mScarlet-NLS-PEST
Plasmid#190658PurposeFluorescent reporter for circadian rhythmsDepositorInsertReverba-mScarlet (Nr1d1 Mouse)
ExpressionMammalianAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only