We narrowed to 49,548 results for: ang

1. TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

   Plasmid

   #221894

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-mGFP

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM30-ARC105

   Plasmid

   #133426

   Purpose

   Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   PCQAP (MED15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains amino acids 5-78 fused to C-terminal of …

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTT5-hSDC1-S15/25A

   Plasmid

   #52364

   Purpose

   expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S15A, S25A and R95Q (according to numbering in Fi…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTT5-hSDC1-N20

   Plasmid

   #52355

   Purpose

   expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of SDC1 aa2-21 (according to numbering i…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCAGGS-Omicron.BA.5-RBD

   Plasmid

   #213478

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/Omicron.BA.5 RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS/EG5-RBD

   Plasmid

   #213410

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/EG5-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-2TK-arc105

   Plasmid

   #133425

   Purpose

   Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   PCQAP (MED15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains amino acids 1-95 or ARC105 fused to the …

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti/TO/EYFP-P2A-IDH1(HA)

   Plasmid

   #122482

   Purpose

   A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

   Depositor

   Eric Huang

   Article

   Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

   Insert

   isocitrate dehydrogenase 1 (IDH1 Human)
   

   Use

   Lentiviral

   Tags

   HA and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTT5-hSDC1-2

   Plasmid

   #52378

   Purpose

   expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GAGAL of syndecan-1 replaced with of GADED of SDC…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLY164

    Plasmid

    #184140

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R3, PrhaB, and Ptet

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY305

    Plasmid

    #184159

    Purpose

    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    K140N, Mutations D10A and H840A on WT cas9. Synon…

    Promoter

    Pcon, PpspA-zraP-22, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTT5-hSDC1-4

    Plasmid

    #52375

    Purpose

    expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GAGAL of syndecan-1 replaced with GDLDD of SDC4; …

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-Lyso-InPTapp

    Plasmid

    #236000

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-InPTapp (PLEKHA1, LAMP1 Human, Synthetic)
    

    Use

    Tags

    Citrine, Full-length LAMP1, and truncECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyso-INPP5E

    Plasmid

    #236004

    Purpose

    Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-INPP5E (LAMP1, INPP5E Human, Synthetic)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-multi-CRISPR-sgRipk1_#1-puro

    Plasmid

    #231984

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-multi-CRISPR-sgRipk1_#2-puro

    Plasmid

    #231983

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

    Plasmid

    #213522

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed V290 to A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

    Plasmid

    #213523

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed N306 to K

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

    Plasmid

    #213524

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to S

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

    Plasmid

    #213525

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to H

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

    Plasmid

    #213526

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed H424 to R

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

    Plasmid

    #221896

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TH0994-pOCC177-Fus-wt_(1-211)

    Plasmid

    #221886

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    PLD domain (aa 1-211)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

    Plasmid

    #221890

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    HNRNPA3 (HNRNPA3 Human)
    

    Use

    Tags

    6xHis-MBP-PreScission-mGFP-TEV

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5-Myc-PSK2 (1-416) (K57A)

    Plasmid

    #197124

    Purpose

    Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

    Insert

    TAOK1 (aa1-416, K57A) (TAOK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A and deleted C-terminus

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLY315

    Plasmid

    #184160

    Purpose

    Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-zntA-1034, PrhaB, and Ptet

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLY162

    Plasmid

    #184138

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R1, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLY163

    Plasmid

    #184141

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R2, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLY168

    Plasmid

    #184142

    Purpose

    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-Ar2, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLY300

    Plasmid

    #184158

    Purpose

    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-oxyS-86, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLY320

    Plasmid

    #184161

    Purpose

    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. JEC027

    Bacterial Strain

    #180310

    Purpose

    E. coli MG1655 strain with knockout of endogenous Type I-E CRISPR Cas system

    Depositor

    James Chappell

    Article

    Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

    Bacterial Resistance

    None

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

35. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. xPERT-hA2q

    Plasmid

    #231063

    Purpose

    Circularly permuted CasRx platform fused to human deaminase domain for A to I editing

    Depositor

    Meng How Tan

    Article

    Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038

    Inserts

    dCas13d
    Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H460D, E488Q, only the deaminase domain (aa 276-7…

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA5-Flag-BRD4-BD

    Plasmid

    #90005

    Purpose

    expresses BD mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 140N to 140A, 433N to 433A

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid

    #229539

    Purpose

    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al bioRxiv November 30, 2024

    Inserts

    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    

    Use

    Tags

    6xHis-3C

    Expression

    Bacterial

    Mutation

    Changed Gly 76 to Val and changed Gly 76 to Val

    Promoter

    T7

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

    Plasmid

    #223959

    Purpose

    Dual fluorescent reporter for histone H2B and for APC/C activity

    Depositor

    Hee Won Yang

    Article

    Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

    Insert

    H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA5-Flag-BRD4-7D

    Plasmid

    #90007

    Purpose

    expresses 7D mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484D, 488S to 488D, 492S to 492D,…

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA5-Flag-BRD4-7A

    Plasmid

    #90006

    Purpose

    expresses 7A mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484A, 488S to 488A, 492S to 492A,…

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pαH-S-GSAS-OMICRON.BA.5

    Plasmid

    #213069

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    S-GSAS-Omicron.BA5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pαH-S-RRAR/XBB.1.16

    Plasmid

    #213037

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    s -RRAR.XBB.1.16 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) 8X His tag 2X…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pαH-S-RRAR-OMICRON.BA.5

    Plasmid

    #213071

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-RRAR-OMICRON.BA.5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685, furin site inta…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEMS1277

    Plasmid

    #29140

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    CAG promoter
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEMS1306

    Plasmid

    #29148

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEMS1307

    Plasmid

    #29149

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEMS1157

    Plasmid

    #29072

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    CAG promoter
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge