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pTK_BsmBI_LacZ_Cherry_Nanotag_105

Plasmid

#130546

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGB

Plasmid

#49203

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

H6-FGB

Tags

His tag + Other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

None

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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POM42-mCherry

Plasmid

#69856

Purpose

N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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gh40

Plasmid

#106714

Purpose

expression of gRNA targeting ST6GAL1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL1 (ST6GAL1 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCOLA kiwi sensor sfGFP

Plasmid

#107368

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

kiwi-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pER-eZinCh-2

Plasmid

#70009

Purpose

expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

ER-eZinCh-2

Tags

KDEL sequence and PPI signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-FOXJ2

Plasmid

#153130

Purpose

Expression of Flag tagged human FOXJ2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXJ2 (FOXJ2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-YL1

Plasmid

#15333

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pTK_BsmBI_LacZ_Cherry_Nanotag_117

Plasmid

#130552

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG

Plasmid

#176545

Purpose

Expresses IgG affinity protein FB with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change glutamic acid 25 to amber stop codon

Promoter

T5

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Tags

pLacZ-ECFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-G

Plasmid

#164162

Purpose

Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-FOXN2

Plasmid

#153138

Purpose

Expression of Flag tagged human FOXN2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN2 (FOXN2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB311

Plasmid

#68639

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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NKP64 GFP (1007)

Plasmid

#62020

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

SLC38A10 (SLC38A10 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-NLS-ZapCmR2

Plasmid

#59012

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR2 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only