We narrowed to 35,847 results for: Met;

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET30b_OmpAT7Sav

Plasmid

#138589

Purpose

T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

Insert

T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export

Use

Synthetic Biology

Tags

OmpA export signal and T7 tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJT1

Plasmid

#98299

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B

Expression

Yeast

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHYX137

Plasmid

#202814

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX138

Plasmid

#202815

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Expression

Yeast

Promoter

ScADH2

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPPC009.106

Plasmid

#171142

Purpose

Expression of J106 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCas2C

Plasmid

#82374

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas34

Plasmid

#82386

Purpose

sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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U6::sgBFP-U6::sgKrasG12D-pX333-red

Plasmid

#149448

Purpose

pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

sgRNA BFP and mouse KRAS G12D

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pV2A-T

Plasmid

#153022

Purpose

Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

Depositor

Vito Valiante

Article

Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPPC017.109

Plasmid

#171145

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPU0 (R)

Plasmid

#83562

Purpose

Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB-BT-Ubiquitin

Plasmid

#36098

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Insert

Ubiquitin (RPS27A Human)

Tags

TB-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBIP1::eYFP

Plasmid

#135231

Purpose

UPR signaling reporter (upregulates eYFP expression under UPR conditions)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

pBiP1

Tags

eYFP

Expression

Plant

Promoter

BiP1

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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