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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,978 results for: Met;

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Showing: 2801 - 2820 of 9978 results

  2. CRY2-INPP5E

    Plasmid
    #79562
    Purpose
    expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  5. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197651-plasmid-map-sequence-id-389657

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  10. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

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  13. Venus1-Y705F/S727A-STAT3

    Plasmid
    #123176
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123176-plasmid-map-sequence-id-238648

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  14. Venus2-Y705F/S727A-STAT3

    Plasmid
    #123177
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123177-plasmid-map-sequence-id-238660

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  15. pICE-EGFP-FLAG-Ku70siR-Mut6E

    Plasmid
    #46962
    Purpose
    For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    Ku70 (XRCC6 Human)
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV Tet
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46962-plasmid-map-sequence-id-66853

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  16. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Ptet(Tetracycline inducible protein)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

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  17. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

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Showing: 2801 - 2820 of 9978 results