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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRubiC-T2A-Cas9

Plasmid

#75347

Purpose

Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR and Retroviral

Tags

mCherry (via t2a)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLOX-CWgfp

Plasmid

#12241

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of GFP. LoxP in 3' …

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDB6 (CK2alpha')

Plasmid

#27084

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-NLS-copGFP-EF1-BlastiS

Plasmid

#132772

Purpose

Spills into the cytoplasm during nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

NLS-copGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa3-IRES-GFP

Plasmid

#33009

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pECFP-IETD2x-Venus

Plasmid

#24536

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-IETD2x-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Mutation

contains an altered Cre gene modified by the addi…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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tetO.Nfia.Blast

Plasmid

#117270

Purpose

Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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CAG sHRPa-NRX

Plasmid

#73143

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Tags

2x HA tag and Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-hspDRv7_scGstlt

Plasmid

#108870

Purpose

scGESTALT V7 lineage barcode for zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Insert

scGESTALT lineage tracing target V7

Use

Tol2 integration into zebrafish genome

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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LMP 1066

Plasmid

#24071

Depositor

Dan Littman

Article

Zhou et al Nature. 2008 May 8. 453(7192):236-40.

Insert

Foxp3 (Foxp3 Mouse)

Use

RNAi and Retroviral

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pD40-His/V5-c-MycT58A

Plasmid

#45598

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc T58A (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Mutation

T58A

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
Arf4-GFP

Plasmid

#39556

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylaion factor 4 (ARF4 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR 13xA (DEPTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG IMP-1-short

Plasmid

#21659

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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