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pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

Plasmid

#20945

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

hSyn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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T7-RelA

Plasmid

#21984

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

NFkB p65 subunit (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

For more details for this expression construct,…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pACBSR-hyg

Plasmid

#87830

Purpose

Expresses lambda-red system with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

bla

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pk7-GR-GFP

Plasmid

#15534

Depositor

Ian Macara

Article

Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

Insert

Glucocorticoid receptor (NR3C1 Human)

Tags

GFP

Expression

Mammalian

Mutation

The glucocorticoid receptor was cloned into the X…

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)

Viral Prep

#162375-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Promoter

Syn

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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