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Plasmid#145116PurposeExpressing base editor A3A-BE3 in yeast cellsDepositorInsertA3A-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT139_GalL_nCDA1-VRERBE3
Plasmid#145102PurposeExpressing base editor nCDA1-VRERBE3 in yeast cellsDepositorInsertnCDA1-VRERBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT92_GalL_nCDA1Δ188-BE3
Plasmid#145052PurposeExpresses nCDA1Δ188-BE3 in yeast cellsDepositorInsertnCDA1Δ188-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-188AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT90_GalL_nCDA1Δ190-BE3
Plasmid#145050PurposeExpresses nCDA1Δ190-BE3 in yeast cellsDepositorInsertnCDA1Δ190-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-190AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT85_GalL_nCDA1Δ193-BE3
Plasmid#145048PurposeExpresses nCDA1Δ193-BE3 in yeast cellsDepositorInsertnCDA1Δ193-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-193AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT84_GalL_nCDA1Δ194-BE3
Plasmid#145047PurposeExpresses nCDA1Δ194-BE3 in yeast cellsDepositorInsertnCDA1Δ194-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-194AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT78_GalL_nCDA1Δ195-BE3
Plasmid#145044PurposeExpresses nCDA1Δ195-BE3 in yeast cellsDepositorInsertnCDA1Δ195-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-195AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LMX1B-gRNA-C-del
Plasmid#131516PurposegRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-delDepositorInsertLMX1B-gRNA-C-del
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 D353A
Plasmid#133016PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
UseTags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationD353APromotert7Available sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 C351A
Plasmid#133014PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
UseTags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationC351APromotert7Available sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorUseTags6xHisExpressionYeastMutationK509APromoterGAL1Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS2
Plasmid#113091PurposeLuciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p bindingDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS1
Plasmid#113090PurposeLuciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding siteDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mCherry Gamma 3
Plasmid#65234PurposeTo study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cellDepositorInsertsUseTags6-His tagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-AVITEV-GFP
Plasmid#74052Purposeretroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInsertAVITEV-GFP
UseRetroviralTagsAVI-TEV-tag N-terminal to the GFPExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-H136T
Plasmid#58413PurposeExpresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationHistidine 236 to ThreoninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_3
Plasmid#36376DepositorInsertMBD4 (MBD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_2
Plasmid#36375DepositorInsertMBD4 (MBD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf199
Plasmid#12887PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf199
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf200
Plasmid#12888PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf200
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf201
Plasmid#12889PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf201
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf202
Plasmid#12890PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf202
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf205
Plasmid#12893PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf205
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf206
Plasmid#12894PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf206
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only