We narrowed to 22,436 results for: Sele

1. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ143-ZmUbi

   Plasmid

   #138107

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

   Plasmid

   #197887

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pEBTet-SNAP-Cep57

   Plasmid

   #136817

   Purpose

   Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep57 (CEP57 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN-CD4

   Plasmid

   #175942

   Purpose

   knockout mouse CD4

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   CD4 gRNA (Cd4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAav-MCS-PQS2-3xHA

   Plasmid

   #84917

   Purpose

   rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Insert

   LOX-PGK-NEO-LOX
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Expression

   Mutation

   Promoter

   mPGK

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDH1-shSHP2-3-H2Kb2

    Plasmid

    #17854

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shSHP2 (PTPN11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPICZ-aC-KHC-BZ

    Plasmid

    #160427

    Purpose

    Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

    Depositor

    Todd Oakley

    Article

    Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    6x Histidine tag, Yeast alpha secretion factor, c…

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTetDBl-CLIP-Cep57

    Plasmid

    #136867

    Purpose

    Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep57 (CEP57 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBTet-SNAP-Cep72

    Plasmid

    #136819

    Purpose

    Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep72 (CEP72 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Flag-Polß(WT)-IRES-Neo

    Plasmid

    #128663

    Purpose

    mammalian expression of Flag-Polß(WT) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC39A14

    Plasmid

    #132105

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGG426_UV5

    Plasmid

    #165608

    Purpose

    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D1135V, S1136R, D1332K and R1333G mutations in Sp…

    Promoter

    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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