Addgene

We narrowed to 10,178 results for: AGA

Showing: 4261 - 4290 of 10178 results

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PCDNA3-N3FLAG-MYB-TAD(251-327)

Plasmid

#105611

Purpose

transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PBK D1.3 gRNA

Plasmid

#90824

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PBK (Guide Designation D1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY3 mutant

Plasmid

#17799

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase

Plasmid

#17800

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
INCENP G3.3 gRNA

Plasmid

#90712

Purpose

3rd generation lentiviral gRNA plasmid targeting human INCENP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

INCENP (Guide Designation G3.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTXB1-xlH2B(1-114)_K114A

Plasmid

#118217

Purpose

Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

xlH2B(1-114)

Use

Tags

Mxe-GyrA intein fused to chitin binding domain

Expression

Bacterial

Mutation

Lysine 114 to alanine

Promoter

T7

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
BUB3 A10.1 gRNA

Plasmid

#90557

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mlp-hCHES1sh

Plasmid

#51845

Purpose

shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

shRNA against CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY1 mutant

Plasmid

#17798

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-GINKO2

Plasmid

#234544

Purpose

to express a potassium sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-GINKO2

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

Plasmid

#234543

Purpose

to express a green fluorescent protein tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-mNeonGreen

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SnoopTag

Plasmid

#225195

Purpose

Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SnoopTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Use

Tags

Expression

Bacterial

Mutation

lexA G85D

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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PGK-HA-PGRN

Plasmid

#234872

Purpose

Lentiviral plasmid expressing HA-tagged human progranulin.

Depositor

Andrew Arrant

Article

Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

Insert

Progranulin (GRN Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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