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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,693 results for: LAS

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Showing: 2841 - 2860 of 42693 results

  1. pLKO_HSV.Ollas.VSVg_NGFR

    Plasmid
    #158274
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.Ollas.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158274-plasmid-map-sequence-id-328330

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  2. Q-ALAS2A-11

    Plasmid
    #166549
    Purpose
    scFv of a human scaffold targeting 5-aminolevulinate synthase, erythroid-specific, mitochondria. Antigen coverage aa 143 - 587 of 587
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166549-plasmid-map-sequence-id-329240

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  5. x.Amylase

    Plasmid
    #87132
    Purpose
    To make in situ hybridization probe for Xenopus amylase.
    Depositor
    Francesca Spagnoli
    Insert
    Amylase
    Expression
    Mammalian
    Available Since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. qTAG-N-Blast-mScarlet-ACTB

    Plasmid
    #207753
    Purpose
    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207753-plasmid-map-sequence-id-416363

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  14. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid
    #207757
    Purpose
    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207757-plasmid-map-sequence-id-415158

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  15. plex_307-3xflag-ccdb-blasticidin

    Plasmid
    #201123
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201123-plasmid-map-sequence-id-396322

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  16. pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

    Plasmid
    #171952
    Purpose
    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3
    Depositor
    Sujatha Jagannathan
    Article
    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:
    Insert
    serine and arginine rich splicing factor 3 (SRSF3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized, truncated at amino acid 114 with…
    Promoter
    TRE
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171952-plasmid-map-sequence-id-338274

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Showing: 2841 - 2860 of 42693 results