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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pzuo1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppfy1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Peno2-ACDH-Ttal1

Plasmid

#127743

Purpose

Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peno2

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Pvma6-ACDH-Trps14a

Plasmid

#127744

Purpose

Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pvma6

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Prpl28-ACDH-Tecm10

Plasmid

#127745

Purpose

Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prpl28

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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drApo1–T7

Bacterial Strain

#156461

Purpose

An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only
lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Article

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-NpHR-EYFP

Plasmid

#171621

Purpose

TRE-NpHR-EYFP reporter

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TRE-NpHR-EYFP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MSP1830

Plasmid

#70708

Purpose

Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Insert

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

CAG

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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BPK764

Plasmid

#65767

Purpose

Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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