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pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-V242K

Plasmid

#136436

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

Depositor

Erik Procko

Publication

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

V242K (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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SFRP1A-c501

Plasmid

#205123

Purpose

SFRP1 protein expression

Depositor

Paul Brennan

Insert

SFRP1 (SFRP1 Human)

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP

Plasmid

#225680

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Publication

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Publication

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)MCS

Plasmid

#31377

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LCMV:GFP(FRT)DsRed

Plasmid

#31378

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)DsRed

Use

Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLEVI(408)-ColE-iLight-msfGFP

Plasmid

#170268

Purpose

Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Tags

msfGFP

Expression

Bacterial

Mutation

To design iLight for expression in bacteria, a fu…

Promoter

pJ23116

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-no-pep

Plasmid

#47439

Purpose

control FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
ARHGEF2A-c055

Plasmid

#205103

Purpose

ARHGEF2 protein expression

Depositor

Paul Brennan

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

6His-TEV

Expression

Bacterial

Mutation

WT

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RHOAA-c035

Plasmid

#205118

Purpose

RHOA protein expression

Depositor

Paul Brennan

Insert

RHOA (RHOA Human)

Tags

6His-TEV and AviTag

Expression

Bacterial

Mutation

WT

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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DAG1A-c003

Plasmid

#205107

Purpose

DAG1 protein expression

Depositor

Paul Brennan

Insert

DAG1 (DAG1 Human)

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1 Catalytic Inactive

Plasmid

#237796

Purpose

Inducible lentiviral expression of RNaseH1 Catalytic Inactive

Depositor

David Pellman

Publication

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Expression

Mammalian

Mutation

D210N

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ADAR1 p110

Plasmid

#237854

Purpose

Lentiviral vector expressing ADAR1 p110

Depositor

David Pellman

Publication

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR1 (ADAR Human)

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Publication

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Apobec3G

Plasmid

#92349

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Publication

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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GST-TPX2(1-43)

Plasmid

#69107

Purpose

For bacterial expression of human TPX2(1-43aa) tagged with GST.

Depositor

Stephen Royle

Publication

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Targeting Protein for Xklp2 (TPX2 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-43 expressed.

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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YFP B1 in pcDNA3.1

Plasmid

#36397

Depositor

Narasimhan Gautam

Publication

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein B1 (GNB1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles