We narrowed to 6,272 results for: KRAS;

Showing: 2881 - 2900 of 6272 results

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AA030

Plasmid

#216010

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-SpG (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa

Plasmid

#193782

Purpose

Express TadCBEa (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa-SpCas9D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pProEx Rab5C(S30C) CL

Plasmid

#224273

Purpose

Protein purification of Rab5C (S30C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rab5C (RAB5C Human)

Expression

Bacterial

Mutation

S30C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Caveolin-C-10

Plasmid

#54025

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AA304

Plasmid

#216072

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-ER-5

Plasmid

#55418

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

ER (CALR Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-cGAS-N-HA

Plasmid

#130913

Purpose

Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS N terminus

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-Venus

Plasmid

#19966

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)

Tags

Flag and monomeric Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only

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AA028

Plasmid

#216008

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA029

Plasmid

#216009

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-NG (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_HOIP/RNF31_#6

Plasmid

#75252

Purpose

retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against human RNF31 (RNF31 )

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

Plasmid

#172501

Purpose

Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 full locus with Golden gate acceptor site for spacer

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_889

Plasmid

#216080

Purpose

ITRs from AAV2, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ITRs from AAV2

Use

AAV; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEe

Plasmid

#193836

Purpose

Express TadCBEe (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDe-SpCas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-S65A

Plasmid

#115619

Purpose

Expresses human Parkin S65A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

S65A

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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