We narrowed to 8,238 results for: TIL;

1. CVS-N2c-jGCaMP8f

   Plasmid

   #172387

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8f
   

   Use

   Neurotropic virus

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

   Plasmid

   #107317

   Purpose

   Expresses SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9
   

   Use

   CRISPR

   Tags

   3xHA and 2xNLS and NLS

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CVS-N2c-nl.EGFP-tdTomato

   Plasmid

   #172382

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + tdTomato
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_VPR_Puro

   Plasmid

   #167887

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HB901: pMVP (L3-L2) polyA

   Plasmid

   #121746

   Purpose

   pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Polyadenylation signal (polyA)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CLM RP HTR

   Plasmid

   #24430

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A

   Available Since

   June 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pOCC193

   Plasmid

   #118903

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease; mGFP and TwinStr…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY-HA1-HA2-neo

   Plasmid

   #109123

   Purpose

   FR cassette provider to introduce FR cassette to a BAC by recombineering

   Depositor

   David Gilbert

   Article

   Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

   Inserts

   HA1
   HA2
   

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LC501: pMVP (L3-L2) mCherry + polyA

    Plasmid

    #121765

    Purpose

    pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP461

    Plasmid

    #85934

    Purpose

    Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 4G7 monoclonal antibody
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-hSyn-Gαi*-BERKY1

    Plasmid

    #158427

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. IZ401: pMVP/Lenti/Neo-DEST

    Plasmid

    #121850

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-merlin 1-339

    Plasmid

    #168128

    Purpose

    Bacterial expression of human Nf2/merlin 1-339

    Depositor

    Tina Izard

    Article

    Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

    Insert

    Merlin (NF2 Human)
    

    Tags

    Glutathione S-transferase (GST)

    Expression

    Bacterial

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_VPR_EYFP

    Plasmid

    #167882

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPU-Empty-miR30-TRIM5

    Plasmid

    #132936

    Purpose

    All-in-one control rescue-TRIM5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    control shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

    Plasmid

    #125227

    Purpose

    Expresses SPARK DRD1 transmembrane component in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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