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We narrowed to 62,256 results for: cloning

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Showing: 2881 - 2900 of 62256 results

  1. R777-E013 Hs.ARHGEF2

    Plasmid
    #70297
    Purpose
    Gateway ORF clone of human ARHGEF2 [NM_001162383.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ARHGEF2 (ARHGEF2 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70297-plasmid-map-sequence-id-125746

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  2. pCIP

    Plasmid
    #79009
    Purpose
    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.
    Depositor
    Reuben Shaw
    Article
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    pCAGGs
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    79009-plasmid-map-sequence-id-151714

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  12. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  17. R777-E011 Hs.ARHGAP35

    Plasmid
    #70295
    Purpose
    Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ARHGAP35 (ARHGAP35 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70295-plasmid-map-sequence-id-125744

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Showing: 2881 - 2900 of 62256 results