We narrowed to 4,276 results for: 424

1. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

   Plasmid

   #174630

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
   

   Use

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGex-4T-2_LC3A

   Plasmid

   #73946

   Purpose

   For recombinant expression of human LC3A/MAP1LC3A in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

3. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5FRT/TO-FAF1-Strep-HA

   Plasmid

   #113486

   Purpose

   Expression of human FAF1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   FAF1 (FAF1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-C-Solo-mStayGold-CLTC

   Plasmid

   #227313

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-MAP3K2

   Plasmid

   #85652

   Purpose

   retroviral expression of human MAP3K2

   Depositor

   Julien Sage

   Article

   Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

   Insert

   MAP3K2 (MAP3K2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF2785

   Plasmid

   #143618

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn GFP-Fxr1

   Plasmid

   #112732

   Purpose

   AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

   Depositor

   Martin Beaulieu

   Article

   Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

   Insert

   Fxr1p (Fxr1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTWIN1-His6-Ssp-Httex1-23Q

   Plasmid

   #84349

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPPI4-Strep-SNAP-CTLA-4-His6

    Plasmid

    #223583

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF3401

    Plasmid

    #144877

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti.PGK.blast-Flag-MFF-WT

    Plasmid

    #74379

    Purpose

    Lentiviral expression vector containing flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid

    #174631

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGex-4T-2_GATE-16

    Plasmid

    #73518

    Purpose

    For recombinant expression of human GATE-16/GABARAPL2 in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    GATE-16 (GABARAPL2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pWPI-LUC7L-3xFLAG

    Plasmid

    #201640

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L (LUC7L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid

    #174633

    Purpose

    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTWIN1-His6-Ssp-Httex1-7Q

    Plasmid

    #84347

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Use

    Tags

    N-terminal His6-tagged intein Ssp

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPU-huMDA5-miR30-MDA5

    Plasmid

    #174224

    Purpose

    All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid

    #113496

    Purpose

    Expression of human Ataxin-3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge