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PUM1_PUM_pGEX

Plasmid

#135129

Purpose

Recombinant expression of PUM1 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PUM1 (PUM1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-ATP

Plasmid

#193818

Purpose

CMV overexpression of ChemoG-ATP in mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-ATP

Expression

Mammalian

Mutation

EGFP:A206K; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA WT

Plasmid

#195202

Purpose

Mammalian expression vector for GFP tagged PKCA.

Depositor

David Bryant, Peter Parker

Publication

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Publication

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Publication

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Publication

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIPZ shTREX1

Plasmid

#127700

Purpose

Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shTREX1 (Trex1 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Mmu

Plasmid

#127698

Purpose

Doxycyclin inducible shRNA knockdown of mouse cGAS gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (Cgas Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Publication

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Publication

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEBB COMMD1 Flag

Plasmid

#74893

Purpose

Express COMMD1 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Publication

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

COMMD1 (COMMD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pVC299 VEGF Site#2

Plasmid

#47506

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA-VEGF site 2 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5FRT PUR mGSK3A K148A

Plasmid

#35158

Depositor

Jim Woodgett

Publication

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Mutation

K148A (dominant negative)

Promoter

CMV

Available since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKanCMV_mRuby3

Plasmid

#172854

Purpose

mRuby3 expressed under the CMV promotor

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mRuby3

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM552

Plasmid

#154131

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Publication

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

wt bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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