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Showing: 2921 - 2940 of 79168 results

  4. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Promoter
    FoxJ1 and human FoxJ1
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  6. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  11. pSW002-Pc-mNeonGreen

    Plasmid
    #205016
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    205016-plasmid-map-sequence-id-424184

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  12. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164423-plasmid-map-sequence-id-338872

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  14. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  15. Tol2-CAG::Palmbow

    Plasmid
    #158993
    Purpose
    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Palmbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158993-plasmid-map-sequence-id-311799

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  16. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid
    #127776
    Purpose
    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus
    Depositor
    Tatjana Sauka-Spengler
    Article
    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517
    Insert
    tfap2b (TFAP2B Chicken)
    Use
    Unspecified
    Tags
    Avi
    Available Since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127776-plasmid-map-sequence-id-250021

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  17. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  20. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid
    #155227
    Purpose
    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Changed Thr 567 to Asp in original Ezrin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155227-plasmid-map-sequence-id-310009

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Showing: 2921 - 2940 of 79168 results