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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PS

Plasmid

#136358

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR

Plasmid

#135500

Purpose

Mammalian expression of FLAG-tagged TAPBPR

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Tags

Luminal FLAG epitope tag and Signal peptide from …

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSG020

Plasmid

#126503

Purpose

Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpGgi with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-proα2(I)-G610C

Plasmid

#119839

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper-PACSIN1-shRNA

Plasmid

#72576

Purpose

Expresses shRNA against PACSIN1

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

PACSIN1 (Pacsin1 Mouse)

Use

RNAi

Expression

Mammalian

Promoter

H1 promoter

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL-CP

Plasmid

#65714

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-282kB mut-LUC

Plasmid

#46867

Purpose

murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

NF- kB site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282C/EBP mut-LUC

Plasmid

#46868

Purpose

murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

CEBP site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
RCAS.Sfi Akt1-K179M

Plasmid

#196375

Purpose

Akt1 kinase negative mutant K179M in avian expression vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

c-akt1 K179M

Use

Retroviral; Avian

Tags

HA

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMA6-TAF4-3xFLAG

Plasmid

#31901

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

TAF4 (Taf4 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Actin 5C

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pMT-Pygopus

Plasmid

#31900

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

Pygopus (pygo Fly)

Tags

6xHIS and V5

Expression

Insect

Promoter

P-MT (Metallothionein)

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
Venus Cam KII alpha 1-315aa

Plasmid

#29431

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

Expresses CamKIIa amino acids 1-315

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only