We narrowed to 10,954 results for: aga

1. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM1157

   Plasmid

   #112534

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL(HrtO) and ProD

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108837

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

   Plasmid

   #108840

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108841

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   PYL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108847

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

   Plasmid

   #108836

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

   Plasmid

   #108842

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GAI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZ_P-GAP-LacI-Mit1AD

   Plasmid

   #126722

   Purpose

   LacI-Mit1AD on pGAPZ_ A expression vector

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   hybrid lacI-Mit1 activation domain coding sequence
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pClneoEGFPC RSF-1

    Plasmid

    #66862

    Purpose

    Express GFP-fused C.elegans RSF-1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    RSF-1 (rsf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S18G, S229T, I403T and F563S mutations in RSF-1 c…

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. KTK_247

    Plasmid

    #180529

    Purpose

    Entry vector containing pTac promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    pTac promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-Scara3-IRES-BFP

    Plasmid

    #117265

    Purpose

    Retroviral overexpression of Scara3

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Scara3 (Scara3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    LTR

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Venus-G protein Gamma 5

    Plasmid

    #42200

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-blast-cfTuba_2

    Plasmid

    #26718

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Canine Tuba_2 RNAi

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGEX2T AtFC1

    Plasmid

    #136363

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana ferrochelatase1

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    Full-length cDNA of Arabidopsis thaliana ferrochelatase1 (FC1 Mustard Weed)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 HRPT2 L64P

    Plasmid

    #11052

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 L64P (CDC73 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L64P

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pY036_ATP1A1_G5

    Plasmid

    #86618

    Purpose

    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

    Plasmid

    #138983

    Purpose

    IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

    Plasmid

    #138990

    Purpose

    IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB3053(gRNA X-2, XI-5, XII-4)

    Plasmid

    #73295

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSuper-PACSIN1-shRNA

    Plasmid

    #72576

    Purpose

    Expresses shRNA against PACSIN1

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Insert

    PACSIN1 (Pacsin1 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TtGNN-TAF4-HFD

    Plasmid

    #106381

    Purpose

    dox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    GFP fused TAF4 HFD (827-909) (Taf4 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMXs-EGFP-ERas delCAAX-IP

    Plasmid

    #13834

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    ERas delCAAX (Eras Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion 4 amino acids of c-terminal end (CAAX mo…

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pMXs-EGFP-ERas C220S/C222S-IP

    Plasmid

    #13829

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    ERas C220S/C222S (Eras Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C220 and C222 changed to serine residues

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA

    Plasmid

    #108838

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    PYL,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108839

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA

    Plasmid

    #108834

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108835

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge