We narrowed to 25,955 results for: Vit;

1. pGEX-4T-3-SH3BP1-CTD

   Plasmid

   #136649

   Purpose

   Expression of SH3BP1-CTD-GST fusion protein in E. coli

   Depositor

   Maria Balda, Karl Matter

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1-CTD (SH3BP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains only C-terminal domain

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-4T-3-SH3BP1-NTD

   Plasmid

   #136648

   Purpose

   Expression of SH3BP1-NTD-GST fusion protein in E. coli

   Depositor

   Maria Balda, Karl Matter

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1-NTD (SH3BP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains only N-terminal domain

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-hSyntenin2a

   Plasmid

   #89436

   Purpose

   mammalian expression of EGFP tagged human Syntenin2a

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

   Plasmid

   #85930

   Purpose

   Sensor Plasmid

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-185-5p Sensor (MIR185 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAC-PHYT

   Plasmid

   #53300

   Purpose

   Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Lyn-AktAR

   Plasmid

   #123356

   Purpose

   FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   Lyn-AktAR
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pASKt15C+SrtA5

   Plasmid

   #64985

   Purpose

   Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, D160N, D165A, K19…

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMiR-E6AP-3'UTR

   Plasmid

   #53694

   Purpose

   Luciferase reporter assay for microRNA binding on E6AP 3'UTR

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   UBE3A 3'UTR (UBE3A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Salsa-BC82-P1G10

    Plasmid

    #202923

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC82
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b+PCNA1 P186C

    Plasmid

    #66173

    Purpose

    Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    P186C

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-mU6-GFPGO-IRES-mScarletI

    Plasmid

    #136895

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-mKOFP2-CAAX

    Plasmid

    #75155

    Purpose

    Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Salsa-BC2-P1A2

    Plasmid

    #202843

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-LIC-N8C_Fab1a

    Plasmid

    #203687

    Purpose

    Expresses N8C_Fab1a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab1a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pIVT-EGFP-mouseCENP-C

    Plasmid

    #174444

    Purpose

    in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Salsa-BC6-P1A6

    Plasmid

    #202847

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hME-pOX

    Plasmid

    #178181

    Purpose

    Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

    Depositor

    Ming-Feng Tsai

    Article

    Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

    Insert

    human MCU-EMRE fusion protein
    

    Use

    Tags

    1D4

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. XE68 APC-GFP in CS2+

    Plasmid

    #16687

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

    Insert

    APC-GFP (APC Human)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

22. FH95pUp95GW (B4)

    Plasmid

    #74013

    Purpose

    lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Use

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-hU6-mU6-Cre2GO

    Plasmid

    #136907

    Purpose

    Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LDB204

    Plasmid

    #185426

    Purpose

    Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 FXFG-repeats domain expressed

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85857

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-21-3p Sensor (MIR21 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HIPK1-myc-his

    Plasmid

    #1850

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    HIPK1 (Hipk1 Mouse)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pTrex-Luc-Phleo

    Plasmid

    #48337

    Purpose

    Trasgenically expresses firefly luciferase in T. cruzi with phleomycin selection

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    firely luciferase
    

    Use

    Trypanasoma cruzi expression

    Tags

    Expression

    Mutation

    Promoter

    T. cruzi rRNA

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAAV-fCAMK-GFP

    Plasmid

    #22917

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGAD424-hSyntenin2b

    Plasmid

    #89427

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    *see below

    Promoter

    ADH1

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pC0.167

    Plasmid

    #119636

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    non functional/large non-specific insertion

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pC0.168

    Plasmid

    #119635

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Down Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 630 A to T

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIRES.Puro.mBeta

    Plasmid

    #24175

    Depositor

    Robert Sobol

    Article

    Jelezcova et al Mutat Res. 2010 Jan 22. ():.

    Insert

    pIRES.Puro.mBeta (Polb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    x

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pBS B2rescue A70D

    Plasmid

    #78658

    Purpose

    Generates B2 inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    B2rescue cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSopB2xHA

    Plasmid

    #112513

    Purpose

    PsopB driven sopB-2xHA and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

    Insert

    sopB-2xHA/sigE operon
    

    Use

    Tags

    2xHA

    Expression

    Bacterial

    Mutation

    SopB C-terminal 2xHA

    Promoter

    PsopB

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET14_GFP5_Encap

    Plasmid

    #86408

    Purpose

    Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 5aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pASKt15C+SrtA7woH

    Plasmid

    #65020

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAT.011

    Plasmid

    #119555

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRL1124

    Plasmid

    #70690

    Purpose

    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Inserts

    avaiM
    eco47iiM
    ecot22iM
    

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge