We narrowed to 3,169 results for: EED

1. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

   Plasmid

   #138985

   Purpose

   IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ihSyn1-DIO-tTA

   Plasmid

   #99121

   Purpose

   An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   DIO-tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG225 HIPK1 3'UTR mut

   Plasmid

   #12044

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   HIPK1 3'UTR mut (HIPK1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pAG177 HIPK1 3'UTR mut

   Plasmid

   #12035

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N10 3'UTR mut (HIPK1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPEDs

   Plasmid

   #162469

   Purpose

   protoplast test vector of pPPED

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Prime editing destination vector for transient ex…

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLuc-CDS-m215-2

    Plasmid

    #42102

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Silent mutations were introduced in the seed-comp…

    Promoter

    SV40

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-gamma2s

    Plasmid

    #118036

    Purpose

    GABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Myc-delta

    Plasmid

    #118037

    Purpose

    GABAA receptor expression (delta subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCGN-HCF-1-C600

    Plasmid

    #92324

    Purpose

    C-term of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 C-term 1436-2035 (HCFC1 Human)
    

    Tags

    C-myc (EQKLISEED) and HA

    Expression

    Mammalian

    Mutation

    AA 1436-2035

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-PCDHGC5

    Plasmid

    #119935

    Purpose

    Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Tags

    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

    Expression

    Mammalian

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Myc-XPLN aa452-526

    Plasmid

    #73381

    Purpose

    Expresses amino acids 452-526 of XPLN (Arhgef3) protein

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Expresses amino acids 452-526 of XPLN (arhgef3) p…

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-Myc-XPLN aa107-311

    Plasmid

    #73379

    Purpose

    Expresses amino acids 107-317 of XPLN (Arhgef3) protein

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Expresses amino acids 107-317 of XPLN (arhgef3) p…

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid

    #128256

    Purpose

    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG179 BC072452 3'UTR mut

    Plasmid

    #12037

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N12 3'UTR mut (FAM107B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only