We narrowed to 8,245 results for: KRAS

1. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HeFSpCas9-A661R

   Plasmid

   #207367

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-evoSpCas9-Q661R

   Plasmid

   #207366

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-Q661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-evoSpCas9-N515Y

   Plasmid

   #207364

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-N515Y
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFL His-I3 V41A / W43A

    Plasmid

    #113515

    Purpose

    Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His

    Expression

    Insect

    Mutation

    V41A /W43A

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE3314

    Plasmid

    #107530

    Purpose

    Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RVR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE3319

    Plasmid

    #107533

    Purpose

    Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3316

    Plasmid

    #107532

    Purpose

    Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE3317

    Plasmid

    #107531

    Purpose

    Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RVR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-mCardinal-TEV

    Plasmid

    #105781

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCardinal-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-mCerulean-TEV

    Plasmid

    #105773

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCerulean-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIX_sh_hMIB2_#9

    Plasmid

    #75255

    Purpose

    retroviral shRNA vector against human MIB2, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against MIB2 (human) (MIB2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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