We narrowed to 9,990 results for: SUB;

1. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a-hRanGAP1

   Plasmid

   #50941

   Purpose

   expression of full length untagged human RanGAP1 in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanGTPase activating protein 1 (RANGAP1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. β3-integrin-CFP

   Plasmid

   #26652

   Depositor

   Jonathan Jones

   Article

   Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

   Insert

   β3-integrin (ITGB3 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1 Flag BRG1

   Plasmid

   #1957

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BRG1 (SMARCA4 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcI-ts,ind+-(NSP8)

   Plasmid

   #160656

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Insert

   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   

   Expression

   Bacterial

   Available Since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-FKBP-PI4KAC1001(D1957A)

   Plasmid

   #139312

   Purpose

   Recruitable PI4KA kinase-dead catalytic fragment

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
   

   Expression

   Mammalian

   Mutation

   D1957A, G1986D

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE DN Cullin5

    Plasmid

    #41916

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL5 (CUL5 Human)
    

    Use

    Lentiviral

    Available Since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4046828

    Plasmid

    #145742

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-AU1-mTOR-E2419K

    Plasmid

    #19994

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-E2419K (Mtor Rat)
    

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Glutamine 2419 to Lysine

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-PD-1 G224A-mGFP

    Plasmid

    #180819

    Purpose

    Expression of human PD-1 G224A fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    G224A

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pyc126m

    Plasmid

    #158777

    Purpose

    ribosome-tagged GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-FKBP-PI4KAC1001

    Plasmid

    #139311

    Purpose

    Recruitable PI4KA catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Expression

    Mammalian

    Mutation

    G1986D

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP‐RNASEH2C

    Plasmid

    #108698

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2C (RNASEH2C Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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