We narrowed to 5,085 results for: mcs

1. pcDNA3.1-3xFLAG-RSL1D1(WT)

   Plasmid

   #122301

   Purpose

   expresses RSL1D1 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZMYND8-dTAG

   Plasmid

   #190617

   Purpose

   Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   ZMYND8 (ZMYND8 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12_G36V and Flag, 2xHA

   Expression

   Mammalian

   Mutation

   synonymous mutation for sgRNA resistance at aa214…

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR-53BP1 1-1972 28A

   Plasmid

   #53447

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Mutation

   I914T mutation; All 28 SQ/TQ motifs in N-term of …

   Available Since

   Dec. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. FLAG-MARK2-CR

   Plasmid

   #229421

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK2-CR (MARK2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   silent mutation CRISPR resistant

   Promoter

   EFS

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

   Plasmid

   #212538

   Purpose

   Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

   Plasmid

   #186740

   Purpose

   Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   RAB10, RABIF (RAB10, RABIF Human)
   

   Use

   Lentiviral

   Tags

   RAB10-T2A-RABIF-P2A-mCherry

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

   Plasmid

   #212572

   Purpose

   Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BAF-WT-tet-puro

    Plasmid

    #199648

    Purpose

    Doxycycline-inducible BAF expression

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    BAF (BANF1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mScarlet-gamma9

    Plasmid

    #140097

    Purpose

    Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    GNGT2
    

    Tags

    N-terminal mScarlet tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Δ18 B.1.351v1

    Plasmid

    #169462

    Purpose

    Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-M-Flag-BAP1-H169Q

    Plasmid

    #125847

    Purpose

    express mutant BAP1-H169Q

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    H169Q

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPc1 hp47phox (S303/304/328D)

    Plasmid

    #112245

    Purpose

    expression of mutant p47phox

    Depositor

    Simone Di Giovanni

    Article

    Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

    Insert

    p47phox (NCF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S303/304/328D

    Promoter

    CMV

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgHcn1#2/Cre

    Plasmid

    #193218

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgHcn1#2 (Hcn1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-Cas9-VQR-Blast

    Plasmid

    #87155

    Purpose

    lentiviral expression of SpCas9-VQR (NGA PAM restricted)

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    SpCas9-VQR(D1135V,R1335Q,T1337R)
    

    Use

    Lentiviral

    Mutation

    D1135V,R1335Q,T1337R

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-NDUFV2-mVenus

    Plasmid

    #185653

    Purpose

    NDUFV2 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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