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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,627 results for: mcs

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Showing: 2961 - 2980 of 5627 results

  1. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  2. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid
    #108372
    Purpose
    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108372-plasmid-map-sequence-id-209438

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  8. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  10. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72259-plasmid-map-sequence-id-131837

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  11. pShuttle-CMV-F2A-T2A-Venus

    Plasmid
    #62621
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
    Depositor
    Zheng-Xu Wang
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62621-plasmid-map-sequence-id-102957

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  12. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid
    #72258
    Purpose
    Express FLPe, copGFP and Puromycin resistance gene under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-copGFp-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available Since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72258-plasmid-map-sequence-id-134986

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  16. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73037
    Purpose
    Express Nluc and copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73037-plasmid-map-sequence-id-136876

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  17. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73022
    Purpose
    Luminescent and fluorescent dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73022-plasmid-map-sequence-id-136024

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  18. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  19. pAAV-TRE-TdTomato

    Plasmid
    #110338
    Purpose
    TdTomato reporter vector of iTango and Cal-Light system for AAV production
    Depositor
    Hyungbae Kwon
    Insert
    tetO-TdTomato
    Use
    AAV
    Expression
    Mammalian
    Promoter
    tetO
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110338-plasmid-map-sequence-id-211133

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  20. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73032
    Purpose
    Express fluorescent and luminescence dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    Truncated EF1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73032-plasmid-map-sequence-id-136029

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Showing: 2961 - 2980 of 5627 results