We narrowed to 13,605 results for: Pol

1. pCMV6-AN-DDK_S520A POLI

   Plasmid

   #131237

   Purpose

   Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_S580A POLI

   Plasmid

   #131238

   Purpose

   Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_3KA POLI

   Plasmid

   #131239

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert lysine resi…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST-pol1-N2-2A2

   Plasmid

   #160991

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGST-pol1-N1-2A2

   Plasmid

   #160990

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-U6+27-Linear-1_PolyU(1)

   Plasmid

   #159479

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Linear-1_PolyU(2)

   Plasmid

   #159480

   Purpose

   Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Linear-1_PolyU(3)

   Plasmid

   #159481

   Purpose

   Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRGLO-POLR2E-213

   Plasmid

   #117356

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR POLR2E-213
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. YCe1889 HC_Kan_SV40-polyA_p11

    Plasmid

    #100678

    Purpose

    part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    SV40-polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Pol-IIΔ

    Plasmid

    #35176

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the entire C-terminal domain (repeats…

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3basic luciferase and renilla_polyA

    Plasmid

    #128046

    Purpose

    pGL3 basic luciferase reporter with co-expression of renilla luciferase

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. POLR2A-N CRISPR pX330

    Plasmid

    #124495

    Purpose

    A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A targeting CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HO-pGAL-poly-KanMX4-HO

    Plasmid

    #51664

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Use

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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