We narrowed to 423 results for: pcag gfp

1. CAG-EGFP-RORbshRNA1

   Plasmid

   #73985

   Purpose

   RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAG-EGFP-RORbshRNA2

   Plasmid

   #73986

   Purpose

   RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

   Plasmid

   #183026

   Purpose

   B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CRY2-GFP-homer1c

   Plasmid

   #89442

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-GFP-homer1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains Full length CRY2

   Promoter

   CAG

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CRY2PHR-PSD95FingR-GFP

   Plasmid

   #89443

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-PSD95FingR-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains CRY2 a.a. 1-498

   Promoter

   CAG

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BLBP-GFP

   Plasmid

   #63174

   Purpose

   Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

   Depositor

   Bart De Strooper

   Article

   Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

   Insert

   BLBP (Fabp7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   1700bp upstream of BLBP gene

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-WIPI2d-TEVcs-TSF

   Plasmid

   #171420

   Purpose

   For the purifcation of GFP- WIPI2d protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GST-GFP-ATG14

    Plasmid

    #171426

    Purpose

    For the purifcation of GFP- PI3KC3 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-TEVcs-GFP-ATG101

    Plasmid

    #171415

    Purpose

    For the purifcation of GFP-ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG101 (ATG101 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid

    #183027

    Purpose

    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

    Plasmid

    #222994

    Purpose

    Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

    Plasmid

    #222992

    Purpose

    Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-DIO_EGFP-GFE3

    Plasmid

    #79871

    Purpose

    For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-DIO_EGFP-RandE3

    Plasmid

    #79872

    Purpose

    For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTAL_GFP

    Plasmid

    #43858

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge