We narrowed to 5,340 results for: 290

1. csd6_13-330_H155A/pET28b(+) N-tag

   Plasmid

   #71357

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Histidine 155 to Alanine)
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

2. csd6_13-330_Y132A/pET28b(+) N-tag

   Plasmid

   #71355

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tyrosine 132 to Alanine)
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pFOSGE_COF4D03

   Plasmid

   #70969

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf12 (Taf12 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-human Igf2bp2-IRES-GFP

   Plasmid

   #91890

   Purpose

   Retroviral expression of human Igfbp2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Igf2bp2 (IGF2BP2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-TetO-hNGN2-Puro

   Plasmid

   #79049

   Purpose

   Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-human LIC1 C-terminal half

   Plasmid

   #74599

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

   Plasmid

   #134645

   Purpose

   lysosome-targeted Sac1 catalytic domain wild type

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   Sac1 catalytic domain (77-520 aa) (SACM1L Human)
   

   Use

   Lentiviral

   Tags

   FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

   Expression

   Mammalian

   Mutation

   contains only amino acids 77-520 (Please see depo…

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgSmarca4#1/Cre

   Plasmid

   #173619

   Purpose

   Expresses a Smarca4-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Smarca4 (Smarca4 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-tetO-NGN2

    Plasmid

    #197090

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2 (NEUROG2 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0978

    Plasmid

    #141775

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-AKAP95-FRB

    Plasmid

    #138254

    Purpose

    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    AKAP95-FRB (AKAP8 Human)
    

    Tags

    FRB rapamycin binding domain and HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2102

    Plasmid

    #141923

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROG2 (NEUROG2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.2_3xFLAG_V5_ mmDdx58_V5

    Plasmid

    #160108

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSynSRE-Mut-T-Luc

    Plasmid

    #60490

    Purpose

    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988

    Depositor

    Timothy Osborne

    Article

    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.

    Insert

    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    

    Use

    Luciferase

    Tags

    Luciferase reporter

    Expression

    Mammalian

    Mutation

    Four point mutations (G->C or A->C) in the …

    Promoter

    Partial HMG-CoA synthase promoter (-324/-225) fus…

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2999

    Plasmid

    #144475

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA GASTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71295

    Purpose

    Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    GASTM mutated glycosylation motif, HA, Lamp2 sig…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid

    #229795

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-GPAT4152-208

    Plasmid

    #134468

    Purpose

    GPAT4152-208 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    GPAT4 (GPAT4 Human)
    

    Expression

    Mammalian

    Mutation

    GPAT4, amino acids 152-208

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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