We narrowed to 8,661 results for: Ott

1. pPP444

   Plasmid

   #158802

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ Dkk1-flag

   Plasmid

   #16690

   Depositor

   Xi He

   Article

   Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

   Insert

   Dkk1 (DKK1 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZRS-IresGFP

   Plasmid

   #21961

   Depositor

   Lynda Chin

   Article

   Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

   Insert

   Ires GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   GACAGATGACTATGCAGAGAT

   Promoter

   Available since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

4. VinculinTS-A50I

   Plasmid

   #111827

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed alanine 50 to isoleucine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP076

   Plasmid

   #158794

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. VinculinTS-I997A

   Plasmid

   #111828

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed isoleucine 997 to alanine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-4xnrUAS

   Plasmid

   #87120

   Purpose

   Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   4xnrUAS (non repetitive)
   

   Use

   Gateway vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL059

   Plasmid

   #177210

   Purpose

   Bicistronic vector with GFP reporter

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBSV2_Psyn-sgRNA500

   Plasmid

   #149613

   Purpose

   gRNA expression in B. burgdorferi, parental vector

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-mKate2-NS

    Plasmid

    #109729

    Purpose

    Multisite gateway vector for 5' tagging with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSAD-F3-NPEST-FLPo-2A-mCherryPEST

    Plasmid

    #99609

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    FLPo
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV5 Smad2-HA

    Plasmid

    #14930

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pPP394

    Plasmid

    #158800

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBSV2_P0026-mCherryBb

    Plasmid

    #118239

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0026 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. myc-mULK1

    Plasmid

    #31960

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (Ulk1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pRRL-Vinculin-Venus

    Plasmid

    #111833

    Purpose

    Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    Vinculin-Venus (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    VenusA206K

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. myc-hULK2

    Plasmid

    #31966

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. p2CT-His-MBP-Hhe_Cas13a_WT

    Plasmid

    #91871

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    HheCas13a (HheC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCyPet-C1

    Plasmid

    #22779

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    CyPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Control for intermolecular Rac1 biosensor FRET. S…

    Promoter

    Available since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pMito.iGABASnFR.F102Y.Y137L

    Plasmid

    #112177

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F102Y.Y137L

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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