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pET-30a(+)-C-SH2+IA

Plasmid

#111418

Purpose

express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1408

Plasmid

#99032

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1928

Plasmid

#99033

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1 Tet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S279A

Plasmid

#60704

Purpose

mammalian expression of rat Cx43 S279A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S279A

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S279D

Plasmid

#60706

Purpose

mammalian expression of rat Cx43 S279D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S279D

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S282A

Plasmid

#60708

Purpose

mammalian expression of rat Cx43 S282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S282A

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S282D

Plasmid

#60710

Purpose

mammalian expression of rat Cx43 S282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S282D

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-E38

Plasmid

#39507

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 E38 (HRAS Human)

Expression

Mammalian

Mutation

G12V and D38E

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pET28aAviHisCD72aCTLD

Plasmid

#129065

Purpose

generation of biotinylated mouse CD72a CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72a (Cd72 Mouse)

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Tau (wt) -VC

Plasmid

#87369

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Tags

c-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-mito-mRaspberry

Plasmid

#219568

Purpose

Lentiviral construct expressing a mitochondria-targeted mRaspberry

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito (COX8A Human)

Use

Lentiviral

Tags

mRaspberry

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

Y705F

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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