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We narrowed to 5,305 results for: Ado

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Showing: 3001 - 3020 of 5305 results

  1. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  4. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

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  5. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  10. pSquash2_SFFV-eGFP-NR3C1-IRES-mCherry_PuroR

    Plasmid
    #247928
    Purpose
    Overexpression vector for eGFP tagged GR or NR3C1. Monitor post-translational degradation of GR via EGFP:mCherry ratio. Contains puromycin-resistance selection marker.
    Depositor
    Benjamin Ebert, Jonathan Tsai
    Article
    Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1
    Insert
    Glucocorticoid Receptor (NR3C1 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    247928-plasmid-map-sequence-id-495611

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  11. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

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  12. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

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  13. pcDNA3.1-GSDMD_29-484

    Plasmid
    #218231
    Purpose
    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-484
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218231-plasmid-map-sequence-id-436177

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  14. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid
    #181893
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181893-plasmid-map-sequence-id-359709

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  15. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

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Showing: 3001 - 3020 of 5305 results