We narrowed to 18,031 results for: Mut

1. HNF4A_P2-285/-169

   Plasmid

   #31052

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   -285 to -1 of P2 HNF4A promoter with mutation -16…

   Promoter

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. HNF4A_P2-285/-136

   Plasmid

   #31051

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   -285 to -1 of P2 HNF4A promoter with mutation -13…

   Promoter

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD10AH840AhCas9 (GB1041)

   Plasmid

   #68207

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tau BA 12->10 N296N

   Plasmid

   #203383

   Purpose

   For transfection and in vitro assay

   Depositor

   Stefan Stamm

   Article

   Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N296N (silent mutation)

   Promoter

   CMVf

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIDTSmart-CMV-PLRS1

   Plasmid

   #217365

   Purpose

   Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   leucyl-tRNA synthetase polyspecific mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M40I, T252A, Y499I, Y527A, H529G

   Promoter

   CMV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdnCas3-CDA1

   Plasmid

   #229535

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   dnCas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdnCas3-APOBEC1

   Plasmid

   #229537

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   dnCas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

   Plasmid

   #191572

   Purpose

   Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   D614G and F486S (confers reduced binding affinity…

   Promoter

   CMV

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM84

   Plasmid

   #220172

   Purpose

   CRISRPi guide RNA against BTF3

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   BTF3 (sgRNA) (BTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.1 R57A, R59A pMT2

    Plasmid

    #206112

    Purpose

    expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S194A, R206H

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S192A, S194A, R206H

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. ACVR1-VSAS-R206H

    Plasmid

    #206185

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    T189V, S192A, R206H

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ACVR1-VASS-R206H

    Plasmid

    #206186

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    T189V, S190A, R206H

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid

    #194890

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid

    #194895

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA2
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

    Plasmid

    #146499

    Purpose

    Insect Expression of DmGW182

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmGW182 (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid

    #194891

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

    Plasmid

    #194896

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_R396A
    U6-crRNA2
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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