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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pMS13

Plasmid

#229474

Purpose

Expresses RssB with Q254A & N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A, N256A)

Expression

Bacterial

Mutation

Q254A, N256A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF42

Plasmid

#229467

Purpose

Expresses RssB with S29C & E229C mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (S29C, E229C)

Expression

Bacterial

Mutation

S29C, E229C

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS26

Plasmid

#229471

Purpose

Expresses RssB with D104N mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104N)

Tags

10xHis

Expression

Bacterial

Mutation

D104N

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS23

Plasmid

#229470

Purpose

Expresses RssB with D104A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Tags

10xHis

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#229462

Purpose

Expresses IraD with Q68A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q68A)

Tags

6xHis

Expression

Bacterial

Mutation

Q68A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMS12

Plasmid

#229461

Purpose

Expresses IraD with W64R mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (W64R)

Tags

6xHis

Expression

Bacterial

Mutation

W64R

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF40

Plasmid

#229459

Purpose

Expresses IraD with R70A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R70A)

Tags

6xHis

Expression

Bacterial

Mutation

R70A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF26

Plasmid

#229458

Purpose

Expresses IraD with Q84A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q84A)

Tags

6xHis

Expression

Bacterial

Mutation

Q84A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234_AE

Plasmid

#148561

Purpose

Bacterial Expression of Hs4EHP_1-234

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234 (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-Hs4EHP_52-234opt_AE

Plasmid

#148566

Purpose

Bacterial Expression of Hs4EHP_52-234opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-52-GB1_AE

Plasmid

#148552

Purpose

Bacterial Expression of HsGIGYF1_33-52

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-52 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71_AE

Plasmid

#148553

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71-GB1_AE

Plasmid

#148554

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-GB1_AE

Plasmid

#148557

Purpose

Bacterial Expression of HsGIGYF1_33-103

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-S99N_AF

Plasmid

#148692

Purpose

Mammalian Expression of Hs4EHP-S99N

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-S99N (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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