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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCI-Neo-HA-MmUbc6/2774

Plasmid

#11482

Depositor

Allan Weissman

Publication

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc6 (Ube2j2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

n/a

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Tags

HaloTag7

Expression

Mammalian

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-Galectin3

Plasmid

#184896

Purpose

Stable expression of HaloTag7-Galectin3 in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

Galectin3

Tags

HaloTag7

Expression

Mammalian

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
MEIS1A-pET

Plasmid

#21015

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Tags

HIS and T7

Expression

Bacterial

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-AktAR2

Plasmid

#64933

Purpose

Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Publication

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

LAMP1-AktAR2 (LAMP1 Synthetic, Budding Yeast, Human)

Tags

Cerulean3, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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f2H ∂HD (272)

Plasmid

#24428

Depositor

Steven Rosen

Publication

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF2 (SULF2 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 418-716 deleted, 23AA N-terminal truncation

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
f1H ∂HD (267)

Plasmid

#24426

Depositor

Steven Rosen

Publication

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 416-726 deleted, 22AA N-terminal truncation

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Publication

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)

Use

Synthetic Biology

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Publication

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Publication

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
FL-BCAN-lentiviral-GFP

Plasmid

#29704

Depositor

Mariano Viapiano

Publication

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

Brevican (BCAN Human)

Use

Lentiviral

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pQUAST-p65AD::CaM

Plasmid

#64715

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Expression

Insect

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKIIK11A::GAL4DBDo

Plasmid

#64722

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::GAL4DBDo

Expression

Insect

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRM102

Plasmid

#44525

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

P(GAL10)-HHT2 P(GAL1)-HHF2

Expression

Yeast

Promoter

GAL 10

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only