We narrowed to 11,027 results for: ENA;

1. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPR v2-sgLKB1 clone1

   Plasmid

   #162125

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2-sgLKB1 clone2

   Plasmid

   #162126

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-parkin WT

   Plasmid

   #45875

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAnc80L65AAP

   Plasmid

   #92307

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

   Depositor

   Luk Vandenberghe

   Article

   Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

   Insert

   Ancestral AAV Capsid Anc80L65AAP
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-CAG-Sun1-GFP-WPRE-pA

    Plasmid

    #220752

    Purpose

    Expression of Sun1GFP for nuclei isolation

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    Sun1-GFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. V5-LOV-Turbo-ERM

    Plasmid

    #199664

    Purpose

    expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    SEC61B and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFLAG_attP

    Plasmid

    #110095

    Purpose

    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AA058

    Plasmid

    #216040

    Purpose

    Fragmid fragment: (C' terminus) for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    MMLV-RT_v1.1; SV40NLS_v1.3
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX208 CMV sTurboID (C)-HA-FRB-ERM

    Plasmid

    #153007

    Purpose

    Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)-ERM (Cb5)
    

    Use

    Lentiviral

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-3xFLAG-TERT

    Plasmid

    #51631

    Purpose

    Mammalian expression of 3xFLAG tagged TERT, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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