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pCherryGFP-CoV2-Inframe-stop

Plasmid

#177620

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-pAce-Kv2.1PR

Plasmid

#195535

Purpose

Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-SC4

Plasmid

#236254

Purpose

AAV-SC4 capsid which targets peripheral nerves in mice

Depositor

Casey Maguire

Article

Abou Haidar et al Gene Ther. 2025 Jun 10. doi: 10.1038/s41

Inserts

rep/cap AAV9 VP1 with GATPSTQ peptide insert
rep

Use

AAV

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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ALFA-bArr1

Plasmid

#201495

Purpose

Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

ALFA-bArr1 (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-DTAG-NTERM-GFP

Plasmid

#185760

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR

Plasmid

#195532

Purpose

Cre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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POLD1_pLX307

Plasmid

#98358

Purpose

Lentiviral expression of POLD1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

POLD1 (POLD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-CTERM-GFP

Plasmid

#185769

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-hTSPAN12

Plasmid

#115784

Purpose

Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12 (TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

multiple silent mutations introduce restriction s…

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo sgTREX1

Plasmid

#127645

Purpose

Knock-out of human TREX1 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

TREX1 sgRNA (TREX1 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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PGK-AID-CTERM-GFP

Plasmid

#185778

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-DTAG-CTERM-GFP

Plasmid

#185765

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

SFFV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-SMASH-NTERM-GFP

Plasmid

#185771

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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