We narrowed to 17,419 results for: She

1. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

   Plasmid

   #180153

   Purpose

   td-sfGFP dre reporter with Rosa26 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Mouse Targeting; Dre/rox

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 Flag-Eya2

   Plasmid

   #49264

   Purpose

   expresses Flag tagged Eya2

   Depositor

   Heide Ford

   Insert

   Eya2 (EYA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pActin5c-CD-dCas9m4-UDI-attB

   Plasmid

   #104879

   Purpose

   To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

   Depositor

   Christopher Potter

   Insert

   CD-dCas9(m4)-UDI
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDS-Red1-N1 AMPA GluR-B(flip)

   Plasmid

   #109142

   Purpose

   The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.

   Depositor

   Stefano Vicini

   Insert

   AMPA receptor
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Glua2 subunit flip spliced form of the AMPA recep…

   Promoter

   CMV

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RAB3B (3DZ8)

   Plasmid

   #32879

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RAB3B (RAB3B Human)
   

   Use

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 18-190 only

   Promoter

   Available since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pLEX307-CTSL-puro

   Plasmid

   #158466

   Purpose

   Constitutive lentiviral expression of human CTSL.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   CTSL (CTSL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-TRAF6-V5-His

   Plasmid

   #63752

   Purpose

   Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   TRAF6 (Traf6 Fly)
   

   Use

   Tags

   V5-His

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

   Plasmid

   #225434

   Purpose

   Transcriptional unit for constitutive expression of the enhanced GFP.

   Depositor

   Diego Orzaez

   Insert

   35S:eGFP:T35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

   Plasmid

   #44085

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   6xGly linker, ADH1 transcription terminator, HphM…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SRPK2

    Plasmid

    #39047

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Internal segment 268-518 deleted; codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pEMB52 / Human_LRRK1_1_360

    Plasmid

    #40549

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Mutation

    WT

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. HaloTag-G protein gamma-12

    Plasmid

    #67043

    Purpose

    G protein gamma-12 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-12 (GNG12 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

    Plasmid

    #121543

    Purpose

    Chronos with red nucleus-targeted fluorescence for inhibitory neurons

    Depositor

    Matthias Prigge

    Insert

    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    dlx5/6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEN_TT CXADR-V5

    Plasmid

    #220358

    Purpose

    Gateway entry vector for an inducible V5-tagged CXADR

    Depositor

    Kevin Janes

    Insert

    CXADR (CXADR Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAS_RvSAHS6

    Plasmid

    #215647

    Purpose

    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PAS_RvSAHS1

    Plasmid

    #215645

    Purpose

    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS1
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG426-GAL-UBQLN2

    Plasmid

    #181708

    Purpose

    Galactose inducible expression of UBQLN2

    Depositor

    Alejandro Chavez

    Insert

    UBQLN2 (UBQLN2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hMYOG-3xHA-IRES-hrGFP (#324)

    Plasmid

    #78341

    Purpose

    Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOG (MYOG Human)
    

    Use

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. cp193g7

    Plasmid

    #25950

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Sci. 2010 Aug;19(8):1490-9. doi:

    Insert

    cp193g7
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1_RapACR_T111C_N248Q-EYFP

    Plasmid

    #119197

    Purpose

    Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

    Depositor

    John Spudich

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C_N248Q
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed threonine 111 to cysteine and asparagine …

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SIRT6

    Plasmid

    #41565

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    aa 3-353

    Promoter

    T7

    Available since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid

    #185060

    Purpose

    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PTPN7

    Plasmid

    #38945

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN7 (PTPN7 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

24. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-synP-FLEX-TVA66T-EGFP-B19G

    Plasmid

    #64097

    Purpose

    Expresses TVA66T-EGFP-B19G under the synapsin promotor

    Depositor

    Ian Wickersham

    Insert

    TVA66T-EGFP-B19G
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glutamic acid 66 to Threonine

    Promoter

    synapsin

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

    Plasmid

    #159902

    Purpose

    Mutagenesis of Slc6a3

    Depositor

    Larry Zweifel

    Insert

    Slc6a3 (Slc6a3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1_hSMAD4

    Plasmid

    #212710

    Purpose

    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    Homo sapiens SMAD4 (SMAD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-7 asyn S87E/S129E

    Plasmid

    #36064

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S87E/S129E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E/S129E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221-CTSL

    Plasmid

    #158444

    Purpose

    Gateway Cloning compatible entry vector for the human CTSL gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSL (CTSL Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid

    #44072

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, His3…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PHGDH

    Plasmid

    #38841

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

33. TLCV2 sgAAVS1

    Plasmid

    #127099

    Purpose

    Encodes gRNA for human AAVS1.

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3-IL-4

    Plasmid

    #40570

    Depositor

    Alexander Dent, Mark Kaplan

    Insert

    IL-4 promoter (Il4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    contains 250 bp of the proximal mouse IL-4 promot…

    Promoter

    mouse IL-4

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pFR022

    Plasmid

    #134228

    Purpose

    bacterial expression of KNL1

    Depositor

    Iain Cheeseman

    Insert

    hKNL1 aa 2106-2316 (KNL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid

    #78332

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

37. hMYF5-3xHA-IRES-hrGFP (#397)

    Plasmid

    #78331

    Purpose

    Overexpression of human MYF5 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid

    #174849

    Purpose

    Expression of DDB1

    Depositor

    Helen Morrison

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PRDM11

    Plasmid

    #32858

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM11 (PRDM11 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 79-314

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits