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pLenti-LbCas12a-8xNLS

Plasmid

#209020

Purpose

Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

LbCas12a-8xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Slc1a3 nickase 1

Plasmid

#129410

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_WT

Plasmid

#82894

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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M3K5_HUMAN_D0

Plasmid

#79710

Purpose

This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

M3K5 (MAP3K5 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
E2AK2_HUMAN_D0

Plasmid

#79712

Purpose

This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

E2AK2 (EIF2AK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DMPK_HUMAN_D0

Plasmid

#79717

Purpose

This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DMPK (DMPK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1G1_HUMAN_D0

Plasmid

#79691

Purpose

This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G1 (CSNK1G1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CSK22_HUMAN_D0

Plasmid

#79704

Purpose

This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK22 (CSNK2A2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1710

Plasmid

#29296

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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TK4_SH4-1_targeting

Plasmid

#254452

Purpose

TK4 targeting vector containing left and right homology arms for the SH4-1 locus

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

mycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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TK4_CLYBL_targeting

Plasmid

#254455

Purpose

TK4 targeting vector containing left and right homology arms for the CLYBL locus

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

mycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐hsRNASEH2BCA

Plasmid

#108692

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A (RNASEH2A Human)

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon a…

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

Plasmid

#108693

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A-D34A/D169A

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon, …

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hERalpha

Plasmid

#101141

Purpose

Mammalian expression of full-length human estrogen receptor-alpha

Depositor

Elizabeth Wilson

Article

Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

Insert

estrogen receptor-alpha (ESR1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

ChR2

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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