We narrowed to 8,227 results for: sars

1. pcDNA3.1-MEF2C-HA

   Plasmid

   #32515

   Depositor

   Andrew Lassar

   Article

   Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

   Insert

   Mef2c (Mef2c Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-E2F4

   Plasmid

   #10914

   Depositor

   Bob Weinberg

   Article

   Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

   Insert

   E2F4 (E2F4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

3. TagBFP_A1aY1

   Plasmid

   #158752

   Purpose

   Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   TagBFP-T_A1aY1
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (CMV enhancer and chicken beta actin promoter)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIDTSmart-MbPylRS

   Plasmid

   #217361

   Purpose

   Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. barkeri pyrrolysyl-tRNA synthetase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSynapsin1-axon-GCaMP6s

   Plasmid

   #111262

   Purpose

   For enriched expression of GCaMP6s in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV1

   Insert

   axon-GCaMP6s
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPML1 (GB0045)

   Plasmid

   #68164

   Purpose

   Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtML1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. myc-Rictor

   Plasmid

   #1860

   Purpose

   Please see Addgene item #11367 for an updated version of this plasmid.

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry_sgRNA-ver2

   Plasmid

   #126776

   Purpose

   gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   sgRNA (for SpCas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Tight-Puro_Nkx2.2

   Plasmid

   #64840

   Purpose

   A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx2.2 (Nkx2-2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6xAP-1_RE::GrRenilla

    Plasmid

    #124534

    Purpose

    AP-1 GreenRenilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    AP-1GreenRenilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pISH-Emx1-SR

    Plasmid

    #105214

    Purpose

    to generate a riboprobe for in situ hybridazation

    Depositor

    Peter Mombaerts

    Article

    Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

    Insert

    Emx1 (Emx1 Mouse)
    

    Use

    To ganerate riboprobe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xDBE_RE::Renilla

    Plasmid

    #124535

    Purpose

    Foxo Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    FOXO Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGB 35S:DsRed:Tnos (GB0361)

    Plasmid

    #68220

    Purpose

    Transcriptional unit for DsRed plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPIK16045_pX330_Flag-SuperFi-Cas9

    Plasmid

    #184370

    Purpose

    Mammalian expression of SuperFi-Cas9 nuclease

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

    Promoter

    CBh

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGrRenilla

    Plasmid

    #118060

    Purpose

    GBPart - GreenRenilla Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Green Renilla
    

    Use

    Luciferase and Synthetic Biology; Gbpart

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Tight-Puro_Olig2

    Plasmid

    #64842

    Purpose

    A lentiviral vector for the expression of mouse Olig2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pbGH

    Plasmid

    #118061

    Purpose

    GBPart - Bovine growth hormone terminator

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    bgH_poly(A)
    

    Use

    Synthetic Biology; Gbpart

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

    Plasmid

    #112007

    Purpose

    For co-expression of GCaMP6s with mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    GCaMP6s-P2A-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-CD4-myc

    Plasmid

    #58537

    Purpose

    Expresses human CD4 with a C-terminal myc tag in mammalian cells

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-hSynapsin1-axon-GCaMP6f

    Plasmid

    #112000

    Purpose

    For enriched expression of GCaMP6f in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV1

    Insert

    axon-GCaMP6f
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-Tight-Puro_ST18

    Plasmid

    #64844

    Purpose

    A lentiviral vector for the expression of mouse ST18 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    ST18 (St18 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcPylRS(Y384F Y306A)

    Plasmid

    #196486

    Purpose

    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hCMV-IE1:GLuc:bgH

    Plasmid

    #135294

    Purpose

    Transcriptional unit for constitutive expression of GLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNLuc

    Plasmid

    #118058

    Purpose

    GBPart - NLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Nano Luciferase
    

    Use

    Luciferase and Synthetic Biology; Gbpart

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. C1-MPAct(delta1-6)-mRuby3

    Plasmid

    #155223

    Purpose

    F-actin bidning mutant of MPAct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Deletion of first 6 AA from F-tractin (9-14 from …

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid

    #155224

    Purpose

    MPAct with increased search radius

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-Tight-Puro_Nkx6.2

    Plasmid

    #64846

    Purpose

    A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx6.2 (Nkx6-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge