We narrowed to 12,310 results for: LEA

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pPS1891

Plasmid

#1363

Depositor

Pamela Silver

Publication

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-EYFP

Expression

Yeast

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pET-hisMBP-rRNaseI

Plasmid

#216673

Purpose

Expresses hisMBP-tagged rat oxidation-resistant RNase inhibitor in E. coli hosts

Depositor

Po-Min Chiang

Publication

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

ribonuclease inhibitor (Rnh1 Rat)

Tags

his-tagged MBP

Expression

Bacterial

Promoter

T7

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCer-Mcm5

Plasmid

#211391

Purpose

mammalian expression of human Mcm5 N-terminally fused to mCerulean

Depositor

Helmut Pospiech

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mcm5 (MCM5 Human)

Tags

mCerulean

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Publication

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-acceptor-2xBbsI

Plasmid

#205633

Purpose

Cloning and U6-drive expression of ISDra2 TnpB reRNAs

Depositor

Gerald Schwank

Publication

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI

Expression

Mammalian

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Publication

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CalfluxCTN

Expression

Mammalian

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUb PspCas13b + PspCas13b guide RNA

Plasmid

#176305

Purpose

Expresses PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Publication

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

E113K, N265S- please depositor comments

Promoter

Ubi-p63e

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-NR4A3

Plasmid

#139818

Purpose

doxycycline-inducible expression of Human NR4A3 in mammalian cells

Depositor

Filipe Pereira

Publication

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

NR4A3 (NR4A3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S240G- please see depositor comment below

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Publication

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGG-NFIX2

Plasmid

#112699

Purpose

Expression of NFIX2 protein and GFP

Depositor

Richard Gronostajski, Francois Guillemot

Publication

Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nuclear Factor I X2 (Nfix Mouse)

Tags

HA

Expression

Mammalian

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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NKP38 GFP (1009)

Plasmid

#62021

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AYTL1 (LPCAT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Publication

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pGPTVII-nlsSED1

Plasmid

#178190

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Publication

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

SV40 NLS

Expression

Plant

Promoter

pUBQ10

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Publication

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p3-K230-FokI_L (wt)

Plasmid

#32544

Depositor

Jin-Soo Kim

Publication

Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

Insert

ZFN

Tags

HA epitope

Expression

Mammalian

Promoter

CMV

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
pET His6 prescission LIC cloning vector (HPKS)

Plasmid

#29720

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Prescission cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Publication

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTN6kwh

Plasmid

#44724

Depositor

Gabor Balazsi

Publication

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

pCMV-D2i promoter
htetR::NLS

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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