We narrowed to 12,130 results for: LEA

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I gamma

Plasmid

#22156

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Dec. 9, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
FLT1-KDR

Plasmid

#86034

Purpose

chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)

Tags

HA and t-GFP

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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hFLT1 delta CTD

Plasmid

#86036

Purpose

human VEGFR1 with deletion of the entire intracellular domain

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 delta CTD (FLT1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

deta CTD

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#223173

Purpose

Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

Plasmid

#176542

Purpose

Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

3' extension derived from endogenous locus a…

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II 15

Plasmid

#35177

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains the C-terminal repeats 16-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-miR29b(m4)

Plasmid

#21122

Depositor

Joshua Mendell

Article

Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

Insert

miR-29b-1 (MIR29B1 Human)

Expression

Mammalian

Mutation

G to C change at nucleotide 21 of the mature miR-…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMT-MCS-NLS-BirA-2A-mCherry_Ras

Plasmid

#80059

Purpose

MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-NLS-BirA-2a-mCherry

Plasmid

#79886

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Promoter

Ubiquitin

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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HisMBP CFlm25

Plasmid

#78458

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie

Article

Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

Insert

Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5R18-CtermQ7

Plasmid

#51443

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pLX304-ARNT

Plasmid

#203578

Purpose

Expresses wild-type ARNT in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

ARNT (ARNT Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11

Plasmid

#179681

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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