Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 17,419 results for: She

Showing: 6161 - 6200 of 17419 results

  1. pFLAG-6a-Ataxin3Q22-C14A

    Plasmid
    #22127
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    C14A
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  2. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPK1 (TPK1 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pDK714

    Plasmid
    #134230
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK365 tdTomato (short SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134230-plasmid-map-sequence-id-272270

    Enlarge

  6. pT7-7 asyn S87A/S129A

    Plasmid
    #36063
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129A (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S87A/S129A
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36063-plasmid-map-sequence-id-44519

    Enlarge

  7. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid
    #78334
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pRK VSV Cortactin

    Plasmid
    #50727
    Purpose
    mammalian expression of VSV-tagged Cortactin
    Depositor
    Kenneth Yamada
    Insert
    Cortactin (CTTN Human)
    Use
    Tags
    2xVSV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

    Enlarge

  10. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

    Enlarge

  11. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

    Enlarge

  12. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180157
    Purpose
    td-sfGFP dre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180157-plasmid-map-sequence-id-355323

    Enlarge

  13. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

    Enlarge

  15. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Expression
    Mutation
    Promoter
    Gal1 promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. PTPN5

    Plasmid
    #38887
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C496S inactivating mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180155
    Purpose
    td-sfGFP cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180155-plasmid-map-sequence-id-355320

    Enlarge

  19. pT7-7 asyn S87A/S129E

    Plasmid
    #36065
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129E (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S87A/S129E
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36065-plasmid-map-sequence-id-44522

    Enlarge

  20. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. RHOU

    Plasmid
    #38885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOU (RHOU Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C191L
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

    Enlarge

  23. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

    Enlarge

  24. pFLAG-6a-Ataxin3Q22-UIM*

    Plasmid
    #22128
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UIM 1,2,3 SA-DG
    Promoter
    Available since
    Nov. 17, 2009
    Availability
    Academic Institutions and Nonprofits only

  25. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

    Enlarge

  26. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

    Enlarge

  27. pUASp YFP Cdc42 WT

    Plasmid
    #52248
    Purpose
    Drosophila expression of YFP-Cdc42
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid
    #52470
    Purpose
    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-aadA-accD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52470-plasmid-map-sequence-id-81333

    Enlarge

  29. FABP9

    Plasmid
    #39107
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FABP9 (FABP9 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. PTGR2

    Plasmid
    #38949
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR2 (PTGR2 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid
    #59282
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    17kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. 4QQN

    Plasmid
    #61951
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L3:APC035-H01:C21507 (PRMT3 Human)
    Use
    Tags
    6xHis-Thrombin and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pCB99

    Plasmid
    #69745
    Purpose
    Retroviral expression of Centrin 2
    Depositor
    Iain Cheeseman
    Insert
    Centrin 2 (CETN2 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. mEos4a-CenpA-C-22

    Plasmid
    #62772
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    NusA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

    Enlarge

  36. AKR7A3

    Plasmid
    #38862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKR7A3 (Akr7a3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. pDK713

    Plasmid
    #134229
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK360 tdTomato (long SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134229-plasmid-map-sequence-id-272275

    Enlarge

  38. 4JDS

    Plasmid
    #60081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7:DCC011-H01:C212434 (SETD7 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. PRKCG

    Plasmid
    #38938
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCG (PRKCG Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6161 - 6200 of 17419 results