We narrowed to 11,027 results for: ENA;

1. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP1-SB100X

   Plasmid

   #154887

   Purpose

   Sleeping Beauty transposase fused to SP1

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   SP1-Sleeping Beauty
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA407

   Plasmid

   #85970

   Purpose

   UPRE-mCh-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   mCherry
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-RhoA-wt

   Plasmid

   #12965

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-RIPK1

   Plasmid

   #78834

   Purpose

   To overexpress RIPK1 in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ER-HA-mNG21-10

   Plasmid

   #141165

   Purpose

   Split Fluorescent Reporter for ER Retrograde Trafficking

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   ER-HA-mNG21-10-KDEL
   

   Use

   Lentiviral

   Tags

   Signal Sequence, KDEL and HA

   Expression

   Mammalian

   Mutation

   K128M, S142T, R150M, G172V and K213M

   Promoter

   Ef1a

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA409

    Plasmid

    #85971

    Purpose

    EF1a-sfGFP-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sfGFP
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-p53/pRK5

    Plasmid

    #39237

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-cytoEKAR4

    Plasmid

    #174438

    Purpose

    Cytosol-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    cytoEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-HA-RIPK3

    Plasmid

    #78804

    Purpose

    To overexpress RIPK3 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

    Plasmid

    #125235

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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