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pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWH-DI

Plasmid

#47385

Purpose

Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

dxs
idi

Expression

Bacterial

Promoter

PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

Lyn

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Promoter

UAS

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T178V, S203A, S207A, S212A

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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CeNL-Actin_pcDNA3

Plasmid

#89512

Purpose

Cyan color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET6

Plasmid

#107438

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET6 (MET6 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(−) mCER-Prx2

Plasmid

#158224

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_iRFP670

Plasmid

#191749

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and iRFP670

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Kohinoor 2.0/pcDNA3

Plasmid

#182315

Purpose

Positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

Insert

Kohinoor 2.0

Tags

No tag

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM2

Plasmid

#35377

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 375-429

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAJM.718

Plasmid

#108519

Purpose

PBetI-YFP Reporter. Cho sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBetI-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-Kan

Plasmid

#44952

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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