We narrowed to 34,850 results for: cas

1. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

   Plasmid

   #149594

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335-sgRNA-EF(Cas9n)-Eif4a1-R

   Plasmid

   #122345

   Purpose

   Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Eif4a1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. RCAS chick Pax6 (DM#226)

   Plasmid

   #15016

   Depositor

   Douglas Melton

   Article

   Kelly et al Dev Dyn. 2000 Aug . 218(4):615-27.

   Insert

   Pax6 (PAX6 Chicken)
   

   Use

   Retroviral; Avian

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pME-nCas9n-P2A-mKate2-pA

   Plasmid

   #109547

   Purpose

   Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   nCas9n and mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PasCas13b-gRNA-EGFP(W58stop)

   Plasmid

   #180507

   Purpose

   PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting EGFP(W58stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PasCas13b-gRNA-Nluc(W12stop)

   Plasmid

   #180506

   Purpose

   PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting Nluc(W12stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CASPR/Neurexin IV scFv [K65/35]

   Plasmid

   #182068

   Purpose

   Mammalian Expression of CASPR/Neurexin IV scFv. Derived from hybridoma K65/35.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting CASPR/Neurexin IV (Rattus norvegicus) (Cntnap1 Mouse)
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.3 CAS9 IRES-Dsred

   Plasmid

   #129444

   Purpose

   expression of FnCas9 and DsRed

   Depositor

   Oskar Laur

   Insert

   FnCas9 IRES-Dsred
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX383 Lactobacillus johnsonii Cas9

    Plasmid

    #68320

    Purpose

    Lactobacillus johnsonii Cas9

    Depositor

    Feng Zhang

    Insert

    LjCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaSpeR4 cyto Grx1-roGFP2

    Plasmid

    #64999

    Purpose

    Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN-PGK-Cas9-WPRE

    Plasmid

    #87886

    Purpose

    To produce Lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    hCas9
    

    Use

    Lentiviral

    Tags

    nls

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS26: pTns(PmcCAST)_ΔTniQ

    Plasmid

    #168159

    Purpose

    Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS27: pTns(PmcCAST)_ΔTnsD

    Plasmid

    #168160

    Purpose

    Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-LmoCas6-KRAB-2a-BlastR

    Plasmid

    #126492

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-KRAB-2a-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC6-Flag CASTOR1 I280A

    Plasmid

    #84497

    Purpose

    Lentiviral expression of CASTOR2 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. SauCas9KKH CCR5-nicking sgRNA

    Plasmid

    #169863

    Purpose

    SauCas9KKH nicking sgRNA for CCR5

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9KKH CCR5-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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