We narrowed to 6,999 results for: nsf

1. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGem-sHyper:sAPX

   Plasmid

   #84738

   Purpose

   Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   sHyper (HyPer2 targeted to nucleus)
   

   Use

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22-His-TEV-DOT1L-MM

   Plasmid

   #89611

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L VVEL293MM Mutant (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)-mRho.V5.mER.hOr47a

   Plasmid

   #126472

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGem-nHyPer:sAPX

   Plasmid

   #84735

   Purpose

   Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   nHyper (HyPer2 targeted to nucleus)
   

   Use

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Mutation

   Promoter

   FMV/35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS L30 Flag-Apex-Rab7A

   Plasmid

   #135652

   Purpose

   Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Apex2 and FLag

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN-D2irTN6kwh

   Plasmid

   #44724

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDam-Myc-HP1

   Plasmid

   #59221

   Purpose

   expresses Dam-myc-HP1

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Insert

   HP1 (Su(var)205 Fly)
   

   Use

   Damid

   Tags

   Dam and myc

   Expression

   Insect

   Mutation

   Promoter

   Drosophila heat shock promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJHG003

    Plasmid

    #126454

    Purpose

    Expresses Cas9-XTEN-dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate TdT.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid

    #123182

    Purpose

    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    

    Use

    Part for plant expression

    Tags

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiex1-SYNAC

    Plasmid

    #82710

    Purpose

    Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

    Insert

    SYNAC (ADCY2, ADCY5 Human)
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CMV-Vcan-V3-HA

    Plasmid

    #238076

    Purpose

    Expresses Vcan V3 in mice heart tissues

    Depositor

    Yuxuan Guo

    Article

    Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

    Insert

    Vcan (Vcan Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKG-GW1-hCIT K42E

    Plasmid

    #203168

    Purpose

    Yeast expression vector for human DHDDS K42E

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    DHDDS (DHDDS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K42E

    Promoter

    TDH3

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22gCfpUbm5En2E4PCh(#251)

    Plasmid

    #184083

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22Ubm5Eng2bE4PCh(#284)

    Plasmid

    #184088

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge