We narrowed to 8,939 results for: pcDNA3

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

Plasmid

#226381

Purpose

Expresses tau propagation ORF with V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-TOPO-mir17-92

Plasmid

#21109

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-17-92 cluster (MIR17 Human)

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-560 USP7

Plasmid

#131244

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 1 - 560. This truncati…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1

Plasmid

#13003

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 (SULF1 Human)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP E2F6

Plasmid

#236441

Purpose

transient overexpression of E2F6 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F6 (E2F6 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-Prx2-mRuby2

Plasmid

#117906

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP E2F4

Plasmid

#236439

Purpose

transient overexpression of E2F4 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F4 (E2F4 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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1478 pcDNA3 flag HA 14-3-3 beta

Plasmid

#8999

Depositor

William Sellers

Insert

14-3-3 beta (YWHAB Human)

Tags

HA and flag

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.4 human alpha4 full

Plasmid

#221397

Purpose

Mammalian expression of human integrin alpha4 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha4 full length (ITGA4 Human)

Tags

Strep twin

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BG4-NLS-mVen

Plasmid

#211449

Purpose

mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

Depositor

Helmut Pospiech

Insert

Anti-DNA G-quadruplex single chain antibody BG4

Tags

NLS-mVenus

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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