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Showing: 3081 - 3100 of 31305 results

  12. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  13. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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  15. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, Human, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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  16. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

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  19. pAAV-phSyn-flex-SypEGFP

    Plasmid
    #153203
    Purpose
    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Lynette Lim
    Insert
    synaptophysin-EGFP
    Use
    AAV
    Promoter
    rat synapsin
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153203-plasmid-map-sequence-id-298916

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  20. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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Showing: 3081 - 3100 of 31305 results