We narrowed to 30,515 results for: Ide

1. pCS2-mkgDUX4mqg*

   Plasmid

   #21175

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Point mutation at nucleotide 6163 (numbering acco…

   Promoter

   Available since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCS2-mkgDUX4mal*

   Plasmid

   #21172

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Point mutation at nucleotide 5114 (numbering acco…

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-X1-Neo-DDRGK1-K-less-HA

   Plasmid

   #139849

   Purpose

   Lentiviral expression of human DDRGK1-K-less-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-eSpCas9

   Plasmid

   #126761

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. eft-3p:act-5(ptc):tbb2 3' UTR

   Plasmid

   #202331

   Purpose

   The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

   Depositor

   Didier Stainier

   Article

   Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

   Inserts

   eft-3 promoter
   act-5(ptc)
   tbb-2 3' UTR
   

   Use

   Tags

   base 238 C -> A mutation causing a PTC at codo…

   Expression

   Worm

   Mutation

   Promoter

   This is a promoter sequence for C. elegans eft-3,…

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ A-SLC-His+Strep-Opa1 (SB254)

   Plasmid

   #227603

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   Opa1
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-960 only

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SAM-DNMT3A-inactive+sgGFP

   Plasmid

   #213169

   Purpose

   Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgGFP
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SAM-DNMT3A-inactive+sgAAVS1_145

   Plasmid

   #213170

   Purpose

   Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_145
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

    Plasmid

    #227606

    Purpose

    Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    R247A,R347A

    Promoter

    AOX1

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ A-MFN2-Strep (SB253)

    Plasmid

    #227602

    Purpose

    Inducible expression of Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    Twin-StrepTag, 10xHis

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1A_Hygro_P2A_RUNX1_R118G_Barcode

    Plasmid

    #170970

    Purpose

    Overexpression of RUNX1 R118G mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1A_Hygro_P2A_RUNX1_N119I_Barcode

    Plasmid

    #170971

    Purpose

    Overexpression of RUNX1 N119I mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N119I

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EF1A_Hygro_P2A_RUNX1_A120D_Barcode

    Plasmid

    #170972

    Purpose

    Overexpression of RUNX1 A120D mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A120D

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF1A_Hygro_P2A_RUNX1_T121I_Barcode

    Plasmid

    #170973

    Purpose

    Overexpression of RUNX1 T121I mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T121I

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EF1A_Hygro_P2A_RUNX1_T101I_Barcode

    Plasmid

    #170956

    Purpose

    Overexpression of RUNX1 T101I mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T101I

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EF1A_Hygro_P2A_RUNX1_L102P_Barcode

    Plasmid

    #170957

    Purpose

    Overexpression of RUNX1 L102P mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L102P

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1A_Hygro_P2A_RUNX1_T104I_Barcode

    Plasmid

    #170958

    Purpose

    Overexpression of RUNX1 T104I mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T104I

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1A_Hygro_P2A_RUNX1_V105E_Barcode

    Plasmid

    #170959

    Purpose

    Overexpression of RUNX1 V105E mutant protein.

    Depositor

    Prashant Mali

    Article

    Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V105E

    Promoter

    Ef1A

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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