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We narrowed to 12,310 results for: LEA

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Showing: 3101 - 3120 of 12310 results

  5. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112060-plasmid-map-sequence-id-216934

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  6. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  7. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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  9. TALEN-CCR5R18-CtermQ7

    Plasmid
    #51443
    Purpose
    Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Right (R18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 KK variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51443-plasmid-map-sequence-id-78480

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  10. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-453897

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  19. pcDNA3-TORCAR-NES

    Plasmid
    #175015
    Purpose
    Cytosol-targeted TORCAR.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175015-plasmid-map-sequence-id-343322

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Showing: 3101 - 3120 of 12310 results