We narrowed to 17,427 results for: emb

1. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKK-mClover3-TEV

   Plasmid

   #105778

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mClover3-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH-CMV-RPS4X-3xHA plasmid

   Plasmid

   #192246

   Purpose

   overexpression vector of RPS4X-3XHA

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   RPS4X CDS without stop coden (RPS4X Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TagBFP2-INPP4BC842A-CAAX

   Plasmid

   #116858

   Purpose

   inactive plasma membrane targetted PI(3,4)P2 phosphatase

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   INPP4B (INPP4B Human)
   

   Tags

   HRAS(172-189) and TagBFP2

   Expression

   Mammalian

   Mutation

   catalytic cysteine 842 changed to alanine

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX VAMP7 (1-188)

   Plasmid

   #42315

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Δ181-220

   Promoter

   Ptac

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

6. TPC1(ΔN69)-mCherry

   Plasmid

   #135184

   Purpose

   TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-69

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKK-TEV-mClover3

   Plasmid

   #105795

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMRX-IP-SECFP-Galectin3

   Plasmid

   #64150

   Purpose

   Expression in mammalian cells (production of retrovirus vector)

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin3 (LGALS3 Human)
   

   Use

   Retroviral

   Tags

   SECFP

   Expression

   Mammalian

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-HemmarG

   Plasmid

   #31221

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Insert

   CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)
   

   Tags

   CD4 and EGFP/GFP

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TPC2(ΔN49)-mCherry

    Plasmid

    #135185

    Purpose

    TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-49

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/CIP2A(1-905) L533E V5 His

    Plasmid

    #119288

    Purpose

    CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    L533E

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKK-TEV-mVenus

    Plasmid

    #105791

    Purpose

    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mVenus

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MBP-FUS_LC_12E

    Plasmid

    #98654

    Purpose

    Expresses MBP-tagged phosphomimetic FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-myc-Miro1 Δ593-618

    Plasmid

    #47895

    Purpose

    Expresses myc tagged Miro1 lacking transmembrane domain

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 Δ593-618 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Δ593-618, lacks transmembrane domain

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pTXB1-EXO1b D173A

    Plasmid

    #68269

    Purpose

    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

    Depositor

    Stefano Ferrari

    Article

    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    catalytically-dead D173A. Please see depositor co…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. mCherry-CRY2-P14P5K

    Plasmid

    #79570

    Purpose

    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pHub10-His-YFP-PIP2;1

    Plasmid

    #99386

    Purpose

    overexpression and isolation of recombinant At-PIP2;1 protein

    Depositor

    Ning Li

    Article

    Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

    Insert

    PIP2;1 (PIP2A Mustard Weed)
    

    Tags

    His and YFP

    Expression

    Plant

    Promoter

    2xCaMV35S

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKK-mEGFP-TEV

    Plasmid

    #105777

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mEGFP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVpuro-Gcn5(D608A)

    Plasmid

    #63707

    Purpose

    retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    D608A mutation

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge